Effectiveness of Haemocoagulase in Wound Healing Process

Singh K.K.¹ , Patle D.R.², U. S. Paikra³

¹Associate Professor, Department of Surgery, Government Medical College, Jagdalpur (C.G.)

²Professor and HOD, Department of Surgery, Government Medical College, Jagdalpur (C.G.)

³Dean, Government Medical College, Jagdalpur (C.G.)

*Corresponding Author E-mail: Drkrishna2272@gmail.com

ABSTRACT:

Background : Wound healing continues to attract great attention of clinical and experimental investigators. There are ample evidence of those unfading interest in the innumerable review that keep coming up in the literature. Aims and objective to evaluate the effectiveness of Haemocoagulase in wound healing processes and make some recommendations if any?

Material and Methods : There were fifty patients admitted for various ailments had to undergo surgical procedures. Haemocoagulase was used for enhancing the wound healing.

Results : There were 08.33% cases where gapping was noted when Haemocoagulase was instilled S/C and in 20.00% when administered I/M. Gapping was lowest i.e. only in 06.25% cases in combined sites. There was no discharge in all the three levels of instillation. It proved that Haemocoagulase when administered in combined sites i.e. I/M + S/C. it is more useful in comparison to alone i.e. either S/C or I/M.

KEYWORDS: Subcutaneous, Intermuscular.

INTRODUCTION:

Haemocoagulase is a purified enzyme complex. Isolated from venome of Brazilian snake "Bothrops Atrox". It promotes and accelerates the physiological process of homeostasis. By appropriate physical and chemical processes, enzymes that accelerates blood coagulation is isolated from the enzyme rich secretion of these snakes. the special manufacturing processes eliminate neurotoxins, haemolysins and other undesirable side effects prothrombin is a protease with a specific action on substrates, similar to that of thrombin itself. However it differs from thrombin that it is not intibited by any of the known thrombin intibitors Blombaeck B. etal (1) and copely et al (2).

The remaining fibrinomers produce polymerization at different optimal conditions of PH and other ionic concentration. the clots thus formed show certain qualitative differences. Since the discovery that thrombin is a Coagulation promoting enzyme research on the clotting mechanism has constantly produced further proof of the activity of this enzyme in the different phases of blood coagulation while thrombin is continuously inactivated in the circulating blood by antithrombins and therefore has no action as long as it is not present in excesses, bothro prothrombin which is not inhibited, possesses an activating effect even at very reduced doses. By judging the above facts authors have undertaken this study to know the effectiveness of Haemocoagulase in wound healing.

MATERIAL AND METHODS:

Fifty patients undergone different operations were taken as sample for this study purposes. Sample contains all age groups and of both sexes. a careful history was taken. Patients operated upon both in routine and in emergency were taken into considerations. all necessary precautions such as all the instruments and material used during operation were sterilized by autoclaving. The sharp instruments were sterilized by Lysol solution. Suture material used were available in pre-sterilised packs. The operative site was shaved wherever necessary and then treated with Savlon betadine solution and spirit. Finally the operation site was drapped with sterilized towels. At the time of closure of wound haemocoagulose was instilled at different site of wound and all sampled cases were divided into three groups (a) Subcutaneous (b) Intermuscular (c) Both intermuscular and subcutaneous. Finally wounds were sutured and dressed with povidone iodine ointment. Wound was schecked for symptoms such as discharge, gapping and healing of wound on 3rd, 7th and 14th day of wound closure i.e. after operation was performed .

OBSERVATIONS AND DISCUSSIONS:

The data thus collected were analyzed and found that 46% of cases were locally instilled with haemocoagulase by subcutaneous level while 28% by intermuscular and in remaining 26% cases it was instilled by both (I/M + S/C) route. (Table-I and Diagram-I) (Table-II and Diagram-II) revealed that through S/C level of instillation 95.83% wound showed healing while in case of both I/M and combined (S/C + I/M) show would healing in 100.00% cases. Thus authors can notice that Haemocagulase more or less equally effective in all three routes though percentage was slightly higher in I/M and in combined i.e, (I/M + S/C).

CONCLUSION:

Thus the authors reached to the conclusion that good surgical techniques plays key role in post operative wound healing. Both local and systemic infection should be handled timely by appropriate antibiotic and correction of systemic problems anaemia uremia and nutritional deficiency etc. Haemocoagulase is helpful in decreasing post operative capillary oozing thus prevents local infection and promotes post operative wound healing by creating adverse atmosphere for infection. Dunphy et al (3) noted that secondary wound healing was entirely depend on local factors not on circulating wound hormones.

TABLE – I DIFFERENT LEVEL AND PROPORTION OF PATIENTS

S.no.	Site of Instillation	Number of cases	Percent
1	Subcutaneous Both	23	46.00
2	Intermuscular	14	28.00
3	(Subcutaneous and Intermuscular)	13	26.00
	Total	50	100.00

TABLE – II CONDITION OF WOUNDS AT DIFFERENT LEVELS

S.No.	Condition of wound	Subcutaneous n = 24	Intermuscular n = 10	Both Subcutaneous + Intermuscular n = 16
1	Discharge	nil	nil	nil
2	Gapping	01 (4.16)	nil	nil
3	Healed	23 (95.83)	11 (100.00)	15 (100.00)
	Total	24 (100.00)	11 (100.00)	15 (100.00)

DIAGRAM – I Number of patients as per site of Instillation of Haemocoagulase

DIAGRAM – II Condition of Wound After Instillation of Feracrylum

REFERENCES:

1. Blombaeck B, Blombaeck M, Nilsson I.M. Coagulation studies on Reptilas, an extract of the venom from Bothrops Jararca, Thromb Diath, Haemorrh I: 1;,1957

2. Gopley A.L. and Luchmi B.W. the binding of human fibrinogen to native and fraction of fibrins and the inhibition of polymerization of a new human fibrin monomer by fibrinogen, life sciences 1293 (1964) Pergamon Press, inc, Printed in USA.

3. Dumphy JE and Jackson DS, Practical application of experimental studies in the case of primarily closed wound. Amer. J Surg 104:273; 1962.

Received on 02.09.2015 Modified on 05.10.2015

Res. J. Pharmacology & P’dynamics. 7(4): Oct.-Dec., 2015; Page 161-162

DOI: 10.5958/2321-5836.2015.00031.2